Fulfill all the requirements listed.

- Baby Daisy – Earn at least a one star ranking in each 50cc Wii Grand Prix cup races

- Baby Luigi – Unlock eight Expert Staff ghost data in Time Trial mode

- Birdo – Defeat 250 people in online racing, or play Time Trial mode on sixteen different courses

- Bowser Jr. – Earn at least a one star ranking in each 100cc Retro Grand Prix cup races

- Daisy – Win the 150cc Special Cup

- Diddy Kong – Win the 50cc Lightning Cup

- Dry Bones – Win the 100cc Leaf Cup

- Dry Bowser – Earn at least a one star ranking in each 150cc Wii Grand Prix cups

- Funky Kong – Unlock four Expert Staff ghost data in Time Trial mode

- King Boo – Win the 50cc Star Cup

- Mii (Outfit A) – To play as the Mii associated with your profile, win the 100cc Special Cup

- Mii (Outfit B) – To play as the Mii associated with your profile in a different outfit, play through and complete all thirty-two time trials

- Rosalina – Have a Super Mario Galaxy save on your Wii

- Toadette – Play Time Trial Mode on all thirty-two courses

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