Fulfill all the requirements listed.

- Baby Daisy - Earn at least a one star ranking in each 50cc Wii Grand Prix cup races

- Baby Luigi - Unlock eight Expert Staff ghost data in Time Trial mode

- Birdo - Defeat 250 people in online racing, or play Time Trial mode on sixteen different courses

- Bowser Jr. - Earn at least a one star ranking in each 100cc Retro Grand Prix cup races

- Daisy - Win the 150cc Special Cup

- Diddy Kong - Win the 50cc Lightning Cup

- Dry Bones - Win the 100cc Leaf Cup

- Dry Bowser - Earn at least a one star ranking in each 150cc Wii Grand Prix cups

- Funky Kong - Unlock four Expert Staff ghost data in Time Trial mode

- King Boo - Win the 50cc Star Cup

- Mii (Outfit A) - To play as the Mii associated with your profile, win the 100cc Special Cup

- Mii (Outfit B) - To play as the Mii associated with your profile in a different outfit, play through and complete all thirty-two time trials

- Rosalina - Have a Super Mario Galaxy save on your Wii

- Toadette - Play Time Trial Mode on all thirty-two courses